It is important, both at national and regional level, the development of markers that allow performing analysis of OO in order to highlighting issues such as quality certification, authentication, geographical and varietal traceability. EVOO traceability is gaining importance due to highquality OO demands, including olive oils with PDO labels or monovarietal ones withspecific characteristic related to the olive variety. There are two essential aspects on EVOO traceability: botanical and geographical ones, that are the core of this proposal. The following objectives can be drawn: 1) development of tools to assess botanical origin of OO; 2)development of tools to assess geographic origin of OO. These tools will allow us to address authentication and certification issues, ensuring that a certain OO is 1) an IGP; 2) a POD; 3) a monovarietal; 4) an organic one; 5) authentic OO (not adulterated with other vegetable oil).